Uncovering the potential on the shelves of drug libraries
Facilitated by DPUK, scientists at the University of Oxford are investigating the behaviour of drugs developed by AstraZeneca (AZ) on the cell models derived from DPUK cohorts. They are looking to see if they can be used to target a range of neurodegenerative diseases, including Alzheimer's and Parkinson's diseases.
This important work started as a meeting of minds at a scientific conference. DPUK’s Stem Cell Network Lead and the Head of Neuroscience Area Therapy at AZ conceived of the idea to re-examine thousands of AZ compounds through a resource that until now has not been available to industry scientists.
State-of-the-art technology helped to secure the partnership
DPUK invested in industry-scale stem cell processing technology at five UK university sites. The highly-automated technologies installed in the Stem Cells Network are now able to efficiently support collaborations with industry in discovering drugs that have therapeutic implications for neurodegenerative disease.
The technology we now have in Oxford vastly improves the efficiency, accuracy and precision of the biochemical testing we carry out here. Now that we are using equivalent technology, it means that industry are willing to engage with the expertise we can offer, and we can pool resources.
- Dr Deepak Kumar, Stem Cell Research Manager, University of Oxford
Working together to make the most of shared resources
DPUK enabled universities and industry to pool the best of their resources: over 4000 compounds and drugs developed by AZ and the DPUK-cohort cell models.
The joint team made use of three key resources. An iPSC stem cell resource was developed by scientists in Edinburgh from the Lothian birth cohort. Following a process known as ‘differentiation’, Oxford-based scientists developed patient-specific neurons outside the human body using optimised methods.
Using the DPUK-funded stem cell technology, scientists at the University of Oxford are carrying out a range of advanced techniques on these patient-specific living brain cells derived from iPSCs. The AZ and university teams are sharing their analysis and processing techniques with each other as they carry out investigations of hundreds of AZ drugs to see if any have any positive effects.
While AZ contributes over 4000 compounds from its drug library, the University of Oxford provides ‘disease in a dish’ models of neurodegenerative disease. Being derived from samples from the DPUK cohorts makes these cells extremely valuable to the scientists. Researchers are able to test the effectiveness of a wide range of drugs provided by AZ in living cells outside the brain. The DPUK stem cell technology is allowing the team to do this more precisely and on a much bigger scale than ever before possible due to the high throughput, automated screening and imaging equipment now available.
There are thousands of drugs that are currently used for other diseases or have been shown to be safe but not treat the disease they were originally designed for. These drugs offer a fantastic opportunity to find new treatments. We’re re-testing thousands of drugs with the DPUK stem cell technology to discover target hits to potentially treat neurodegenerative diseases. - Dr Brent Ryan, OPDC Career Development Fellow, University of Oxford